Studies on Oral Microorganisms : 4 Gram Stain by Thin-layer-agar Plating

Analytical studies on regeneration of protoplasts of Geotrichum candidum by quantitative thin-layer-agar plating.

A preparation of pure protoplasts of Geotrichum candidum became osmotically stable and colonies developed when the protoplasts were embedded in stabilizing thin-layer-agar and incubated with stabilizing basal medium. When growing protoplasts were exposed to distilled water and then reincubated with basal medium, the process of regeneration of protoplasts could be quantitatively demonstrated by ...

Streptococcal pharyngitis--rapid diagnosis by Gram stain.

Group A beta-haemolytic streptococci were isolated from 51 (9.8%) of 519 patients with pharyngitis. Throat culture results showed the mean sensitivity, specificity and predictive value of a positive Gram-stained smear of pharyngeal secretions as 70%, 89% and 69%. The data suggest that Gram staining of a smear directly from the swab is as accurate as and more speedy than a culture from the phary...

Campylobacter enterocolitis diagnosed by stool Gram stain

A 5-year-old boy with no past medical history presented to the emergency department with a 4-day history of low-grade fever, abdominal pain, and watery diarrhea with intermittent mild hematochezia. Seven days before presentation, he had attended a family barbecue and had consumed chicken that was slightly raw. His family members showed no symptoms despite consuming the same chicken. On physical...

The Gram stain.

Gram-Positive Rods on a Cerebrospinal Fluid Gram Stain

Cerebrospinal fluid (CSF) access device placement in the pediatric population presents challenges due to the development of infections following placement, access or revision, and/or shunt malfunctions. Here we report an unusual pediatric case of L. monocytogenes ventriculitis/VP shunt (VPS) infection and associated pseudocyst with an emphasis on the importance of VPS removal in clearing the in...